Inhibitory Power of Mouthwash Containing Pineapple Cobs (Ananas Comosus (L.) Merr.) Ethanol Extract toward the Growth of Streptococcus Mutans
Abstract
Aim: This study aimed to investigate the inhibitory power of mouthwash containing pineapple cobs ethanol extract with concentrations of 7,5%, 10%, 15%, 20%, 25%, and 30% against Streptococcus mutans.
Materials and Methods: The agar well diffusion method was employed in order to investigate the inhibitory power of mouthwash containing pineapple cob extract against the growth of Streptococcus mutans. A Few (20-100 µl) volumes of the mouthwash containing pineapple cobs ethanol extract from each of the concentrations was placed into each of the wells on blood agar in petri dishes had contained Streptococcus mutans then incubated for 24 hours at 370C temperature and after these the measurement of inhibition power was measured with a caliper.
Results and conclusions: The results showed that mouthwash containing pineapple cobs ethanol extract with concentrations of 7,5%, 10%, 15%, 20%, 25%, and 30% could inhibit the growth of Streptococcus mutans. Mouthwash containing Pineapple cobs extracts with concentrations of 7,5%, 10%, and 15% have moderate inhibition power, and mouthwash containing pineapple cobs extracts with concentrations of 15%, 20%, 25%, and 30% have strong inhibition power. The conclusion is mouthwash containing pineapple cobs ethanol extract has inhibitory power against Streptococcus mutans.
References
Mulyanti S, Laela DS, Julaeha E, Surawagiani AA, Aripin. Formulation of mouth rinse from the essential oils of lime (Citrus aurantifolia) and its inhibitory efficacy on the growth of Streptococcus mutans – in vitro. Padjadjaran Journal of Dentistry.2020; 32(1):39-47. doi: https://doi.org/10.24198/pjd.vol32no1.25486
Litbang Kemkes. Laporan nasional Riset kesehatandasar 2018. Jakarta. http://repository.bkpk.kemkes.go.id/id/eprint/3514
Lemos JA et al. The biology of Streptococus mutans, Microbiol Spectr. 2019, 7(1). doi: https://doi.org/10.1128/microbiolspec.gpp3-0051-2018
Samarayanake, Lakshman. Essential Microbiology for Dentistry. 4th ed. Elsevier; 2012. https://evolve.elsevier.com/cs/product/9780702072178?role=student
Ningsih W, Afdhil Arel. Clove Oil (Syzgium aromaticum) Edible Film Formulation and Antibacterial Activity Test against Streptococus mutans. Journal of Fundamental and Applied Pharmaceutical Science. 2021; 2(1). doi: https://doi.org/10.18196/jfaps.v2i1.11640
Abdurrahman M, Lestari ES, Prihatiningsih T. The Effectiveness of Ethanol Extract Neem Leaf (Azadirachta indica) Mouthwash against the growth of Streptococus sp. Journal of Biomedicine and Translational Research. 2022; 8(2):76-80. doi: https://doi.org/10.14710/jbtr.v8i2.14732
Yuslianti et al,. Peningkatan Pengetahuan Bahan Alamuntuk Kesehatan Gigi dan Muilut Melalui Program Merdeka Belajar Kampus Merdeka. Jurnal inovasi dan Penerapan Ipteks. 2022; 10(1). doi: https://doi.org/10.18196/berdikari.v10i1.13480
Minarni, Rosmalia D. Uji Daya Hambat Antibakteri Ekstrak Bonggol Nanas terhadap Bakteri Streptococcus mutans. Jurnal kesehatan. 2022; 13(1):159-163. doi:https://doi.org/10.35730/jk.v13i1.707
Minarni,.Pengaruhber kumurdengan Maserasi Ekstrak Bonggol Nanas terhadap PH Saliva Rongga Mulut. Jurnal Kesehatan Gigi 6. 2019.63-67. doi: https://doi.org/10.31983/jkg.v6i1.4435
Ouchari L, Boukeskasse A, Bouizgarne B, Ouhdouch Y. Antimicrobial Potential of Actinomycetes isolated from the unexplored hot merzouga desert and their taxonomic diversity. Biology Open. 2019; 8. doi: https://doi.org/10.1242/bio.035410
Pelczar MJ, Chan ECS,. Dasar Dasar Mikrobiologi. 2th ed. Jakarta. Universitas Indonesia Press; 2008.
Lasmayanti, Metty, Potensi Antibakteri Propolis Lebah Madu Trigona Sppterhadap Bakteri Kariogenik (Streptococcus mutans). FMIPA ITB. 2007.
Nugraha PY, Astuti ESY, Shinta NM,. Pineapple Weevil extract (Ananas Comosus (L Merr). Inhibits S.mutans in child dental caries. Makassar Dental Journal. 2021; 10(3):273-278. doi: https://doi.org/10.35856/mdj.v10i3.464
Jancic U, Gorgieva S. Bromelain and Nisin: The Natural Antimicrobial with High Potential in Biomedicine. Pharmaceutics Journal. 2022; 14(76). doi: https://doi.org/10.3390/pharmaceutics14010076
Umarudin, Sari RE, Fal B, Syukrianto, Efektivitas Daya Hambat Ekstrak Etanol 96% Bonggol Nanas (Ananas Comosus (L) Merr) terhadap Pertumbuhan Bakteri Staphylococcus aureus. Journal of Pharmacy and Science.2018; 3(2). doi: https://dx.doi.org/10.53342/pharmasci.v3i2.114
Cushnie, TPT, Lamb AJ. Review antimicrobial activity of flavonoids. International journal of antimicrobial agents 26. 2005:343-356. doi: https://doi.org/10.1016/j.ijantimicag.2005.09.002
Nuria MC, Faizatun A, Sumantri. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Jarak Pagar (Jatropas curcas L) terhadap Bakteri Staphylococcus auerus ATCC 25923, Escherichia coli ATCC 25922, Dan Salmonelatyphi ATCC 1408. Mediagr. 2009; 5(2):26-37. doi: http://dx.doi.org/10.31942/mediagro.v5i2.559
Cowan MM. Plant Products as Antimicrobial Agents. Linivalm Microbiology Reviews. 1999; 12:564-582. doi: https://doi.org/10.1128/cmr.12.4.564
Suliani A, Latief M, Rahmi SL. Aktivitas Antimikroba Ekstrak Etil Asetat Buahdan Daun Belimbing Wuluh (Averhoa Bilimbi Linn) Terhadap Mikroba Salmonela typhimurium dan Aspergillus flavus. Chempublish Journal. 2016; 1(2):32-41; https://online-journal.unja.ac.id/chp/article/view/3311