Evaluation of the Efficacy of Laser, Desensitizing Tooth Paste and Mouthwash on Obliteration of Dentinal Tubules: An in Vitro Scanning Electron Microscopic Study
Background: The aim of this study is to evaluate and compare the efficacy of laser, desensitizing toothpaste, and desensitizing mouthwash on dentinal tubular obliteration using scanning electron microscopy (SEM).
Materials and Methods: Thirty extracted, sound, single-rooted mandibular premolars were selected. The enamel was removed with tungsten carbide fissure bur and crown dentin discs, with a thickness of 2 mm, which was cut perpendicular to the long axis of the tooth. Each specimen was etched with 37% phosphoric acid for 30seconds to expose the dentinal tubules. Specimens were again rinsed with distilled water and ultrasound to remove the residual smear layer for five minutes. Group I – Application of Arginine Calcium Carbonate containing toothpaste, Group II – Application of Potassium sorbate, and Cetyl Pyridinium Chloride containing mouthwash, Group III – Application of 810nm diode laser in noncontact mode. All samples were processed and examined under a scanning electron microscope (SEM) to compare the effectiveness of dentinal tubule obliteration.
Results: The mean values of percentages of dentinal tubule obliteration in Groups I, II, III were 2.20, 4.60, 1.10 respectively. Diode laser group (Group III) was found to be more effective in causing dentinal tubule obliteration followed by the toothpaste group (Group I) and mouthwash group (Group II). Conclusion: Within the limitations, the laser group was found to be more effective in dentinal tubule obliteration when compared to the desensitizing toothpaste group and the desensitizing mouthwash, which was statistically significant (P<0.001).