Effectiveness of Rosella Flower Extract (Hibiscus Sabdariffa L.) in Gel and Liquid form on the Growth of Streptococcus Mutans Bacteria

  • Vitri Nurilawaty Lecturer, Department of Dental Health, Poltekkes Kemenkes Jakarta I, Indonesia.
  • Dwi Priharti Lecturer, Department of Dental Health, Poltekkes Kemenkes Jakarta I, Indonesia.
  • Ai Emalia Sukmawati Lecturer, Poltekkes Kemenkes Jakarta II, Indonesia.
  • Tedi Purnama Lecturer, Department of Dental Health, Poltekkes Kemenkes Jakarta I, Indonesia.
Keywords: Gel, Liquid, Rosella Flower Extract, Streptococcus Mutans

Abstract

Background: The most dominant bacteria in dental caries-causing plaque is Streptococcus mutans bacteria, which is a type of acid-producing bacteria that can damage teeth due to the fermentation reaction of carbohydrates including sucrose, fructose, and glucose. Inhibiting the growth of these bacteria is an alternative way to inhibit the growth of plaque so as to prevent dental and gum disease. One of the herbal plants that can be used as an alternative medicine as an anti-bacterial is rosella flower (Hibiscus sabdariffa L.)

Material and Methods: The research design used is Experimental. The research was conducted from July to November 2021 at the Pharmacognosy Laboratory, Department of Pharmacy and Food Analysis, Poltekkes Kemenkes Jakarta II, and QLab, Faculty of Pharmacy, University of Pancasila. The research sample was rosella flower extract in gel and liquid preparations in concentrations of 5%, 10%, 15%, 20%, and 30%. Data analysis using Repeated Measure Anova and Independent Sample Test.

Results: Rosella flower extract was effective in inhibiting the growth of streptococcal mutans bacteria, where the gel preparation had a p-value of 0.000 (p<0.05) and a liquid preparation with a p-value of 0.003 (p<0.05). The average zone of inhibition of the growth of Streptococcus mutans bacteria in gel preparations was greater than in liquid preparations in each concentration. The zone of inhibition for gel preparations at 30% concentration was 14.4 mm, while for liquid preparations it was 10.7 mm. The smallest inhibition zone was gel preparation at a concentration of 5% of 10.8 mm, while the smallest liquid preparation was at a concentration of 10% at 6.8 mm.

Conclusion: Rosella flower extract was effective in inhibiting the growth of streptococcus mutans bacteria and rosella flower extract in gel form was greater than liquid in inhibiting the growth of streptococcus mutans bacteria.

References

RE PR, Purnama T, Lestari SY. Informed Consent to Patients in Root Canal Treatment (Case Study: Melati Dental Clinic Jakarta, Indonesia). Medico-Legal Updat. 2021; 21(1). doi: https://doi.org/10.37506/mlu.v21i1.2411

Kidd EAM, Fejerskov O. Essentials of dental caries. Oxford University Press; 2016. https://global.oup.com/academic/product/essentials-of-dental-caries-9780198738268?cc=us&lang=en&

Davis IJ, Wallis C, Deusch O, Colyer A, Milella L, Loman N, et al. A cross-sectional survey of bacterial species in plaque from client owned dogs with healthy gingiva, gingivitis or mild periodontitis. PLoS One. 2013; 8(12):e83158. doi: https://doi.org/10.1371/journal.pone.0083158

Qiu W, Zhou Y, Li Z, Huang T, Xiao Y, Cheng L, et al. Application of antibiotics/antimicrobial agents on dental caries. Biomed Res Int. 2020; 2020. doi: https://doi.org/10.1155/2020/5658212

Yadav K, Prakash S. Dental caries: A microbiological approach. J Clin Infect Dis Pr. 2017; 2(1):1–15. doi: https://doi.org/10.4172/2476-213X.1000118

Hiranya MP, Eliza H, Neneng N. Ilmu pencegahan penyakit jaringan keras dan jaringan pendukung gigi. Jakarta EGC. 2011; 104.

Montaser R, Luesch H. Marine natural products: a new wave of drugs? Future Med Chem. 2011; 3(12):1475–89. doi: https://doi.org/10.4155/fmc.11.118

Putri RM, Diana VE, Fitri K. Perbandingan uji aktivitas antibakteri dari ekstrak etanol bunga, daun dan akar tumbuhan rosella (Hibiscus sabdariffa L.) terhadap bakteri Staphylococcus aureus. J Dunia Farm. 2019; 3(3):131–43.

Wiyarsi A. Khasiat bunga rosella (Hibiscus sabdariffa L). Univ Negeri Yogyakarta Yogyakarta. 2011;

Suarti BS, Ardilla D, Jubeir A. Studi Pembuatan Selai Bunga Rosella (Hibisscus sabdariffa L.). AGRIUM J Ilmu Pertan. 2015; 17(1).

Nugroho CA. Aktivitas hipoglikemik ekstrak rosela (Hibiscus sabdariffa L.) pada tikus putih diabetes. Widya War J Ilm Univ Katolik Widya Mandala Madiun. 2010; 34(01):1–9.

Ulung G, Studi P. Sehat Alami dengan Herbal: 250 Tanaman Berkhasiat Obat. Vol. 1. Gramedia Pustaka Utama; 2014.

Haidar Z. Si Cantik Rosella: Bunga Cantik Berjuta Khasiat. Edumania; 2016.

Nurilawaty V, Hanum NA, Wahyuni S. Pengaruh air seduhan kelopak bunga rosella(hibiscus sabdariffa l.) Terhadap kelarutan ionkalsium gigi permanen ( in Vitro). J Pembang Mns. 2013; 7(3). https://ejournal.sumselprov.go.id/pptk/article/view/266

Wulandari BD. Pengaruh Pemberian Seduhan Kelopak Bunga Rosella (Hibiscus Sabdariffa) Dosis Bertingkat Selama 30 Hari terhadap Gambaran Histologik Ginjal Tikus Wistar. Faculty of Medicine; 2010. http://eprints.undip.ac.id/23599/

Muspahuddin M. Pengaruh Metode Pengeringan Kelopak Bunga Rosella (Hibscus sabdariffa L.) terhadap Aktivitas Antioksidan. Universitas Islam Negeri Alauddin Makassar; 2011. http://repositori.uin-alauddin.ac.id/19489/

Published
2022-05-01
How to Cite
Nurilawaty, V., Priharti, D., Sukmawati, A. E., & Purnama, T. (2022). Effectiveness of Rosella Flower Extract (Hibiscus Sabdariffa L.) in Gel and Liquid form on the Growth of Streptococcus Mutans Bacteria. International Journal Of Drug Research And Dental Science, 4(2), 1-9. https://doi.org/10.36437/ijdrd.2022.4.2.A